Bleached (M state) D96N crystal after brief exposure to yellow light
Click here for a quicktime movie showing three cycles of
Note: The file is large (28 MBytes). During the first 11 seconds
the microscope is being focused. The sequence was recorded at beamline
7-1 at the Stanford Synchrotron Radiation Laboratory (SSRL).
Structural Changes in Bacteriorhodopsin During Ion Transport at 2
H Luecke, B Schobert, H-T Richter, JP Cartailler, JK Lanyi (1999) Science 286, 255-260.
The atomic models:
1c8r.pdb (released Oct 12, 1999): 1.8 Å light-adapted ground-state structure of the D96N single-site mutant, described in the 1999 Science paper.
(released Oct 12, 1999): 2.0 Å M photocycle-intermediate structure
of the D96N single-site mutant, described in the 1999
A few related web pointers:
Perspective Article by R. Gennis & T. Ebrey:
Research Article (pages 255-260):